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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be observed in many tumors, which leads to chromosome instability, and thus could lead to tumorigenesis. This protein was identified to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial part in advertising and marketing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two supplemental splice variants ended up described but in-depth nucleotide sequence info is not really nevertheless available.
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Blocking peptides are peptides that bind particularly for the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized from the antibody.
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This guide outlines more doable optimization of cellular and immunoassay parameters to make sure the very best results are...
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This manual outlines even more doable optimization of mobile and immunoassay parameters to make sure the absolute best effects are...
Antibodies sure to the blocking peptide now not bind to your epitope on the goal protein. By comparing the staining with the blocked antibody compared to the antibody by itself, you can see which staining is distinct.
Target Facts Cyclin E belongs for the hugely conserved cyclin spouse and children, whose users are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation designs which lead on the temporal coordination of every mitotic function. Cyclin E kinds a fancy with and features like a regulatory subunit of CDK2, whose action is required for cell cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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